P-236: The Role of Trace Elements in Treatment of Ovariectomized Osteoporotic Rats

Background: Osteoporosis is one of the uncomfortable postmenopausal symptoms. The risk of imbalanced nutrition especially traces elements and vitamins are high during post menopause and may lead to osteoporosis due to reduction of content of mineral bone. The aim of this study was to investigate the potential consequence of Selenium (Se) treatment in ovariectomized rat model with osteoporosis in preventing bone loss. Material and Methods: 30 mature female Wistar rats weighing approximately 200g were selected and ovariectomized. In control group rats were given distilled water and in experimental group were gavaged with different doses of Sodium selenite, (0.1 -0.6 mg/kg). It was administrated for two months. The femur bone of the animals was collected and tissue bone investigated under a light and electron microscope level. Results: The ovariectomized rats showed a significant decrease in bone mass density of femur in comparison with the intact control group. Ovariectomized group not treated by Se showed inappropriate lamellar structure and a large uncalcified bone matrix. Administration of Se rat reversed bone loss and prevented destruction of bone tissue and bone trabecular formation and arrangements were observed in the treated group and had similar structure with control group compared to the ovariectomized group. Serum biochemical assays revealed that Se prevent osteoporosis induced in ovariectomized group (p<0.05). Conclusion: Se prevents structural alternation in bone after osteoporosis. Bone resorption was considered as an effective therapeutic approach. These data clearly suggest that Se has the potential effect as an alternative treatment for prevention of bone loss in postmenopausal osteoporosis.